Functional Consequences of Lipid-Mediated Interaction Between Rhodopsin Molecules

INTERACTION OF PROTEINS WITH SMALL MOLECULES

c-Met and Other Cell Surface Molecules: Interaction, Activation and Functional Consequences

The c-Met receptor, also known as the HGF receptor, is one of the most studied tyrosine kinase receptors, yet its biological functions and activation mechanisms are still not fully understood. c-Met has been implicated in embryonic development and organogenesis, in tissue remodelling homeostasis and repair and in cancer metastasis. These functions are indicative of the many cellular processes i...

The rhodopsin-transducin complex houses two distinct rhodopsin molecules.

Upon illumination the visual receptor rhodopsin (Rho) transitions to the activated form Rho(∗), which binds the heterotrimeric G protein, transducin (Gt) causing GDP to GTP exchange and Gt dissociation. Using succinylated concanavalin A (sConA) as a probe, we visualized native Rho dimers solubilized in 1mM n-dodecyl-β-d-maltoside (DDM) and Rho monomers in 5mM DDM. By nucleotide depletion and af...

Consequences of interaction between opioid and receptor.

Gaddum (1962) divided receptors into ‘pharmacological’ and others. Pharmacological receptors give a pharmacological response to binding of molecules of drug (including endogenous substances) and are blocked by specific antagonists. One such is the opiate receptor. Its ligands include opiates (derivatives or opium), opioid drugs (e.g. methadone, pethidine), opioid peptides (endorphins, including...

Interaction between molecules of hantavirus nucleocapsid protein.

Intermolecular interactions of Tula hantavirus N (nucleocapsid) protein were detected in the yeast two-hybrid system, prompting further attempts to study this phenomenon. Using chemical cross-linking and immunoblotting it was shown that the N protein from purified virus and from infected cell lysates as well as recombinant protein produced in a baculovirus expression system are capable of formi...